Silica induced early fibrogenic reaction in lung of mice ameliorated by Nyctanthes arbortristis extract.
Biomed Environ Sci
; 15(3): 215-22, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12500662
ABSTRACT
OBJECTIVE:
To investigate the pharmacological effect of Nyctanthes arbortristis (NAT) leaf extract in the prevention of lung injury induced by silica particles.METHOD:
Lung injury was induced in Swiss mice through inhalation exposure to silica particles (< 5 mu) using a Flow Past Nose Only Inhalation Chamber at the rate of -10 mg/m3 respirable mass for 5 h. Lung bronchoalveolar lavage (BAL) fluid collected between 48 and 72 h was subjected to protein profiling by electrophoresis and cytokine evaluation by solid phase sandwich ELISA. Lung histopathology was performed to evaluate lung injury.RESULTS:
Inhalation of silica increased the level of tumor necrosis factor-alpha (TNF-alpha), and of the 66 and 63 kDa peptides in the BAL fluid in comparison to sham-treated control. Pre-treatment of silica exposed mice with NAT leaf extract significantly prevented the accumulation of TNF-alpha in the BAL fluid, but the 66 and 63 kDa peptides remained unchanged. The extract was also effective in the prevention of silica-induced early fibrogenic reactions like congestion, edema and infiltration of nucleated cells in the interstitial alveolar spaces, and thickening of alveolar septa in mouse lung.CONCLUSION:
NAT leaf extract helps in bypassing silica induced initial lung injury in mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibrose Pulmonar
/
Silicose
/
Dióxido de Silício
/
Exposição por Inalação
/
Oleaceae
/
Fitoterapia
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomed Environ Sci
Ano de publicação:
2002
Tipo de documento:
Article