[Plant regeneration from protoplast culture in flax (Linum usitatissimum)].
Shi Yan Sheng Wu Xue Bao
; 33(2): 163-9, 2000 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-12548980
ABSTRACT
Shoot protoplasts of four fiber flax (Linum usitatissimum) varieties (7309, 948, Belinka and Viking) were isolated and cultured. The optimal condition for higher protoplast yield 1.8 x 10(6)/gFW and activity 85.5% (c.v. 948) were from 10 day old seedings. Culture in V-KM Agroase-island medium led to first divisions after 3 days (c. v. 948), and after twenty days with an efficiency of 36% of divided cells and 5.2% in plating efficiency. Plant regeneration was obtained in 7309 and Belinka on agar media B5-2, MS3 containing 0.6 mg/L 6-BA and 0.1 mg/L NAA. Roots and leaves regeneration were observed in Viking and 948 respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Protoplastos
/
Linho
Idioma:
Zh
Revista:
Shi Yan Sheng Wu Xue Bao
Ano de publicação:
2000
Tipo de documento:
Article