[Study on biochemical mechanism of citral damage to the A. flavasi's mitochondria].

ABSTRACT

Adopted biochemical method and combinded with Scanning Electron Microscope(SEM) to observe, study the inhibition-growth mechanism to A. flavus when citral was permeated to the cell and damaged mitochondria, the result suggested that the mitochondria changed in many kinds of unrule shape and the oxidation-reduction system was destroyed, when the citral reached at the level of anaphylactic consistency to A. flavus. Compared with the control group, activities of MDH and SDH in experimental group non-reversibly decreased 27.1% and 23.8% respectively, and grandully lost. When respectively used the succinate, pyruvate and alpha-ketoglutarate as substrates, the respiration speed of mitochondria separatelly decreased 24.1%, 36.1%, 14.3%. It suggested that the citral could inhibit the biosynthesis of the mycelial DNA, RNA, lipoid and protein, so promoted to dath.