Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV-haemorphin-7 (LVVh-7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor.
Biotechnol Appl Biochem
; 37(Pt 3): 317-24, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-12558504
ABSTRACT
Peptic hydrolysis of native bovine haemoglobin at pH 3 yields the LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; LVVh-7) opioid peptide corresponding to the residues-31-40 fragment of the beta-chain of haemoglobin. This peptide is intermediate in the course of batch hydrolysis and is rapidly degraded. Indeed, it shows an optimum at 3% degree of hydrolysis (i.e. 2 min of reaction time). The hydrolysis was carried out in a continuous membrane reactor with a space time (ratio of the flux to the reactor volume) set to 2 min (corresponding to optimum LVVh-7 production). This process allows the continuous production of a constant fraction of intermediate peptides containing LVVh-7 for 48 min.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Hemoglobinas
/
Ultrafiltração
/
Peptídeos Opioides
/
Reatores Biológicos
/
Membranas Artificiais
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Biotechnol Appl Biochem
Ano de publicação:
2003
Tipo de documento:
Article