Detection of bovine leukemia virus in blood and milk by nested and real-time polymerase chain reactions.
J Vet Diagn Invest
; 15(1): 72-6, 2003 Jan.
Article
em En
| MEDLINE
| ID: mdl-12580302
ABSTRACT
Concerns about retroviruses in livestock and products derived from them have necessitated the development of tests to detect the bovine leukemia virus (BLV) in blood and milk from cattle. Dairy cattle (n = 101) from 5 different geographical areas were used for this study. A nested polymerase chain reaction (PCR) identified 98% of BLV seropositive cattle (n = 80) from blood and 65% from milk, whereas real-time PCR detected 94% of BLV seropositive cattle from blood and 59% from milk. Bovine leukemia virus was also detected by PCR in approximately 10% of seronegative cattle (n = 21), most likely because of early detection before seroconversion.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sangue
/
Reação em Cadeia da Polimerase
/
Leucose Enzoótica Bovina
/
Vírus da Leucemia Bovina
/
Leite
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Ano de publicação:
2003
Tipo de documento:
Article