Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum.
Vet Immunol Immunopathol
; 92(1-2): 87-94, 2003 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-12628766
ABSTRACT
Infection with Ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. In the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (L3) with significant reductions in both worm (P<0.03) and faecal egg counts (P<0.0004) following a challenge infection. Vaccination with irradiated L3 and challenge with infective L3 stimulated a dominant antibody response to antigens of less than 20 kDa in an excretory/secretory extract from adult parasites. Immunoscreening of an adult A. caninum cDNA library with antisera from the vaccine trial identified a number of clones. The three clones with the strongest immunoreactivity proved to be identical and encoded a peptide with similarity to the N-terminal domain of the tissue matrix metalloproteinase inhibitor (TIMP)-2 mammalian tissue metalloproteinase inhibitor family.
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Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
/
3_ND
Base de dados:
MEDLINE
Assunto principal:
Vacinas Atenuadas
/
Vacinação
/
Doenças do Cão
/
Ancylostoma
/
Ancilostomíase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
2003
Tipo de documento:
Article