A molecular method for the recovery and identification of enteric virus in shellfish.
New Microbiol
; 26(2): 157-62, 2003 Apr.
Article
em En
| MEDLINE
| ID: mdl-12737197
ABSTRACT
In this paper we report the results of an investigation into the presence of enteric viruses in shellfish from the waters around Sardinia. Twenty two samples of shellfish were examined using a rapid and sensitive technique to concentrate and detect viral RNA in shellfish tissues. After recovery of viral particles, RNA was extracted, transcribed into cDNA and amplified using "nested PCR". Testing with enterovirus-specific RT-PCR produced positive results in over 13% of specimens. The virus detection procedure appears to be effective. In some circumstances it could be a better test of water quality than conventional monitoring techniques.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Frutos do Mar
/
Enterovirus
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
País/Região como assunto:
Europa
Idioma:
En
Revista:
New Microbiol
Ano de publicação:
2003
Tipo de documento:
Article