Production of ajmalicine and ajmaline in hairy root cultures of Rauvolfia micrantha Hook f., a rare and endemic medicinal plant.
Biotechnol Lett
; 25(8): 631-6, 2003 Apr.
Article
em En
| MEDLINE
| ID: mdl-12882157
ABSTRACT
Hairy roots of Rauvolfia micrantha were induced from hypocotyl explants of 2-3 weeks old aseptic seedlings using Agrobacterium rhizogenes ATCC 15834. Hairy roots grown in half-strength Murashige & Skoog (MS) medium with 0.2 mg indole 3-butyric acid l-1 and 0.1 mg alpha-naphthaleneacetic acid l-1 produced more ajmaline (0.01 mg g-1 dry wt) and ajmalicine (0.006 mg g-1 dry wt) than roots grown in auxin-free medium. Ajmaline (0.003 mg g-1 dry wt) and ajmalicine (0.0007 mg g-1 dry wt) were also produced in normal root cultures. This is the first report of production of ajmaline and ajmalicine in hairy root cultures of Rauvolfia micrantha.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rauwolfia
/
Ioimbina
/
Raízes de Plantas
/
Alcaloides de Triptamina e Secologanina
/
Ajmalina
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2003
Tipo de documento:
Article