[Study on the transcriptional activation of MTS1 gene beta promoter].
Zhonghua Xue Ye Xue Za Zhi
; 24(7): 344-6, 2003 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-12941186
ABSTRACT
OBJECTIVE:
To investigate the effect of MTS1 gene beta promoter transcriptional activation in T-cell acute lymphoblastic leukemia (T-ALL) cell lines and identify the fragment with transcriptional activation.METHODS:
Seven pGL3 recombinant plasmids with the same 3'-end transcriptional start site but the different 5'sequences were constructed by gene recombinant technique and transfected into Jurkat cell line which is biallelic deletion of MTS1 gene by transient transfection. Luciferase report gene was detected to observe beta promoter transcriptional activation.RESULTS:
Seven pGL3 recombinant plasmids containing different fragments of beta promoter were obtained, all of them showed transcriptional activation in Jurkat cell line. Among them, the 0.38 kb fragment cut by SacII-SacI is fundamental in transcription.CONCLUSION:
MTS1 gene beta promoter can be activated in Jurkat cell line.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Transcricional
/
Regiões Promotoras Genéticas
/
Genes p16
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Xue Ye Xue Za Zhi
Ano de publicação:
2003
Tipo de documento:
Article