[The expression of mRNA and SDS-PAGE of L1210 cell strains and its cloned cells].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 34(2): 207-9, 2003 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-12947690
ABSTRACT
OBJECTIVE:
To examine the expression difference of mRNA of L1210 cell strains and its cloned cells and discuss the methods for quality control of cell strains.METHODS:
We used SDS-PAGE to observe the difference of protein and performed in situ hybridization to examine the expression of mRNA with the use of 6 cDNA probes that were marked by biotin.RESULTS:
The number of protein bands of L1210 from Beijing Cancer Institute was 32. The number of protein bands of the two cloned cells L3E11 and L3F9 was 31. The 6 cDNA probes (p16, c-fos, c-jun, c-myc, p21, and p53 mRNA) were found to be existing in Beijing Cancer Institute L1210 and two different cloned cell strains. Expression of c-myc, c-fos, p53 mRNA could distinguish L3E11 and L3F9 cloned cells.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia L1210
/
Proteína Supressora de Tumor p53
/
Proteínas de Neoplasias
Limite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2003
Tipo de documento:
Article