Selection of a human chromosome 21 enriched YAC sub-library using a chromosome-specific composite probe.
Nat Genet
; 1(4): 284-90, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1302025
ABSTRACT
The subdivision of total genomic human yeast artificial chromosome (YAC) libraries into specific chromosome clone collections will greatly facilitate the construction of an integrated genetic, physical and transcriptional map of the genome. We report the isolation of 388 YAC clones from a human library with an average insert size of 620 kilobases (kb) by the hybridization of a composite chromosome 21 probe to a high-density array of YAC clones. Roughly half of these clones hybridize to chromosome 21 by fluorescence in situ hybridization. These clones represent a twofold coverage of the chromosome. The technique offers the potential of sub-dividing whole genomic YAC libraries into their chromosomal elements to produce high-resolution tools for genome mapping.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 21
/
Biblioteca Gênica
/
Genoma Humano
Limite:
Humans
Idioma:
En
Revista:
Nat Genet
Ano de publicação:
1992
Tipo de documento:
Article