[The use of nucleolytic enzymes (ribonucleases, polynucleotide phosphorylases and endonuclease from Serratia marcescens) for producing initial blocks of synthetic endoribonucleases]. / Primenenie nukleoliticheskikh fermentov (ribonukleaz, polinukleotidfosforilaz, éndonukleazy Serratia marcescens) dlia polucheniia iskhodnykh blokov sinteticheskikh éndoribonukleaz.
Nauchnye Doki Vyss Shkoly Biol Nauki
; (2): 9-15, 1992.
Article
em Ru
| MEDLINE
| ID: mdl-1338866
ABSTRACT
The simplest variant of synthetic substrate-ribozyme complex has been proposed. The schemes of potential ribozyme "subunits" synthesis have been worked out R1--GCUUGAAACAAA; R2--AAAAACUGAUGAAAGC. The macroscale synthesis of dinucleoside monophosphate ApU, GpC, CpU catalyzed by immobilized ribonucleases of different specificity and preparation of oligoadenylates by hydrolysis of poly-A in the presence of endonuclease Serratia marcescens, as well the synthesis of conservative sequences of potential ribozyme such as ApUpG, CpUpG, GpApU, ApApApG and others have been described.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Polirribonucleotídeo Nucleotidiltransferase
/
Ribonucleases
/
Serratia marcescens
/
Endonucleases
/
Endorribonucleases
Idioma:
Ru
Revista:
Nauchnye Doki Vyss Shkoly Biol Nauki
Ano de publicação:
1992
Tipo de documento:
Article