Use of the Escherichia coli SSB gene to prevent bioreactor takeover by plasmidless cells.
Biotechnology (N Y)
; 8(1): 47-51, 1990 Jan.
Article
em En
| MEDLINE
| ID: mdl-1366512
ABSTRACT
Reactor takeover by plasmidless cells is a major problem encountered when producing proteins from plasmid-borne genes in genetically engineered bacteria. We have approached this problem by deleting the essential ssb gene from the Escherichia coli chromosome and placing it on a plasmid. Plasmidless cells do not accumulate even after growing such strains under non-selective continuous culture conditions for extended periods of time. Other ssb-containing plasmids can be readily introduced into this E. coli strain by a plasmid-displacement technique. Using this system, we have achieved very high levels of beta-lactamase production in continuous culture without selective pressure.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Proteínas de Ligação a DNA
/
Escherichia coli
/
Genes Bacterianos
Idioma:
En
Revista:
Biotechnology (N Y)
Ano de publicação:
1990
Tipo de documento:
Article