Purification, characterization, and amino terminal sequence of xylose reductase from Candida shehatae.
Enzyme Microb Technol
; 12(1): 33-9, 1990 Jan.
Article
em En
| MEDLINE
| ID: mdl-1367448
ABSTRACT
D-Xylose is a major component of the carbohydrates derived from agricultural residues and forest products. Among more than two hundred known xylose-utilizing yeasts, only a few species are known to be able to ferment xylose anaerobically. Candida shehatae is one of such xylose-fermenting yeasts. Xylose reductase (E.C. 1.1.1.21) is a key enzyme responsible for xylose metabolism in xylose-utilizing as well as xylose-fermenting yeasts. In this paper, we report the development of a convenient and reliable procedure for the purification of xylose reductase from C. shehatae to near homogeneity. The amino acid composition and N-terminal sequence of the enzyme have also been analyzed. C. shehatae seems to contain only a single xylose reductase, but the enzyme has a dual coenzyme specificity for both NADPH and NADH. The enzyme is remarkably stable at room temperature and 4 degrees C.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desidrogenase do Álcool de Açúcar
/
Candida
/
Aldeído Redutase
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
1990
Tipo de documento:
Article