Mercury in a commercial preparation of rat amylin.
Pept Res
; 5(3): 161-4, 1992.
Article
em En
| MEDLINE
| ID: mdl-1421804
ABSTRACT
The biological activity of amylin is reported to vary widely depending on the source and purity of the material. Three commercial samples of rat amylin were compared for structural differences. The samples were nearly identical using most of the available analytical measures--amino acid analysis, HPLC retention, even Edman sequencing data. When the samples were compared by ion spray ionization mass spectrometry, the molecular mass of one sample was 200 daltons higher than anticipated. Careful analysis of the sample, including atomic emission spectrometry, revealed that a mercury atom was associated with the polypeptide. The mercury presumably resulted from a deprotection step in the synthesis, involving the removal of an acetamidomethyl group from cysteine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Amiloide
/
Mercúrio
Limite:
Animals
Idioma:
En
Revista:
Pept Res
Ano de publicação:
1992
Tipo de documento:
Article