Kinetic control of 5 S RNA gene transcription.
J Mol Biol
; 227(4): 1009-18, 1992 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-1433283
ABSTRACT
We have determined that the differential transcription of somatic and oocyte-type 5 S RNA genes in a Xenopus laevis oocyte extract is a consequence of vastly different rates of stable complex assembly. Somatic-type 5 S RNA genes sequester a limiting transcription factor much more rapidly than oocyte-type 5 S RNA genes. Once formed, however, transcription complexes on both types of genes are stable, and are transcribed at nearly equivalent rates. The relative rates of stable transcription complex assembly are strongly dependent on the concentration of Mg2+. Kinetic differences in transcription complex assembly provides a key distinguishing feature between these two genes which may be used in the selective repression of oocyte-type 5 S RNA genes during the early development of Xenopus, and may also be utilized in other systems of regulated gene expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
RNA Ribossômico 5S
/
Regulação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1992
Tipo de documento:
Article