Improving multiple isomorphous replacement phasing by heavy-atom refinement using solvent-flattened phases.
Acta Crystallogr A
; 48 ( Pt 5): 751-6, 1992 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-1445683
ABSTRACT
Solvent flattening of macromolecular MIR electron density maps is frequently used to improve the quality of the phases and the interpretability of resultant electron density maps. A new method is presented by which the heavy-atom parameters of isomorphous derivatives are refined against these same solvent-flattened phases and is shown to enhance convergence of the parameters by decoupling heavy-atom-parameter adjustment from parent-phase calculation. This approach is described here in the first example of its application in the solution of the glutaminyl-tRNA synthetase-tRNA(Gln)-ATP co-crystal structure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Difração de Raios X
/
RNA de Transferência de Ácido Glutâmico
/
Glutamato-tRNA Ligase
Idioma:
En
Revista:
Acta Crystallogr A
Ano de publicação:
1992
Tipo de documento:
Article