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Dengue virus type 2 infects human endothelial cells through binding of the viral envelope glycoprotein to cell surface polypeptides.
Wei, Hui-Yong; Jiang, Li-Fang; Fang, Dan-Yun; Guo, Hui-Yu.
Afiliação
  • Wei HY; Medical Laboratory Center, Guangdong Provincial People's Hospital, 106 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
  • Jiang LF; Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
  • Fang DY; Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
  • Guo HY; Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou 510080, People's Republic of China.
J Gen Virol ; 84(Pt 11): 3095-3098, 2003 Nov.
Article em En | MEDLINE | ID: mdl-14573814
The endothelial cell line ECV304, derived from human umbilical cord and identified to be susceptible to dengue virus type 2 (DEN-2) infection, was used to study the molecular mechanism of DEN-2 binding to endothelial cells. DEN-2 was found by virus overlay protein-binding assays (VOPBAs) to bind to three ECV304 cell membrane proteins with molecular masses of 29, 34 and 43 kDa. Only a single protein of 29 kDa was observed when VOPBAs were carried out using preparations of trypsin-treated ECV304 cells. Pre-incubation of live ECV304 cells in culture or cell membrane proteins in modified VOPBAs with the recombinant DEN-2 envelope glycoprotein (rEgp) inhibited DEN-2 infection and blocked virus binding to the three proteins identified. These results indicate that DEN-2 rEgp could bind to three proteins on the surface of ECV304 cells. This virus-cell interaction may be associated with the receptor complex specific for DEN-2 infection of endothelial cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Receptores Virais / Endotélio Vascular / Proteínas do Envelope Viral / Proteínas de Membrana Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Gen Virol Ano de publicação: 2003 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Receptores Virais / Endotélio Vascular / Proteínas do Envelope Viral / Proteínas de Membrana Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Gen Virol Ano de publicação: 2003 Tipo de documento: Article