Is the cytomegalovirus serologic status always accurate? A comparative analysis of humoral and cellular immunity.
Transplantation
; 76(8): 1229-30, 2003 Oct 27.
Article
em En
| MEDLINE
| ID: mdl-14578758
ABSTRACT
BACKGROUND:
The precise knowledge of cytomegalovirus (CMV) status is important in a variety of clinical settings such as transplantation or blood transfusion. There are, however, various situations where an immunoglobulin-based diagnostic approach has limitations.METHODS:
The CMV status of 388 individuals was determined by the analysis of both CMV-specific immunoglobulins and cellular immunity using a standard enzyme-linked immunosorbent assay and a rapid-flow cytometric approach, respectively.RESULTS:
In 386 of 388 individuals, both assays yielded concordant results in assigning the CMV status (294 positive and 92 negative). In 2 of 94 (2.1%) CMV immunoglobulin (Ig) G-negative individuals, CMV-specific CD4 T cells were detectable. Repeated testing of the index and follow-up samples yielded one positive and one marginally positive IgG titer.CONCLUSIONS:
Both assays yielded highly concordant results. Thus, the T-cell-based approach may represent a reliable alternative for defining CMV status in any clinical situation where serologic testing is limited by borderline titers or by the presence of passively administered antibodies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citomegalovirus
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Adult
/
Aged
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Humans
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Middle aged
Idioma:
En
Revista:
Transplantation
Ano de publicação:
2003
Tipo de documento:
Article