Lack of correlation between telomere length and telomerase activity and expression in leukemic cells.
Int J Mol Med
; 12(6): 935-8, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14612969
ABSTRACT
The expression of three components of telomerase complex (hTR, hTERT, TP1) along with telomerase activity and telomere length in leukemic cells was investigated. Cells were isolated from peripheral blood and/or bone marrow of children with acute lymphoblastic (ALL) and non-lymphoblastic (ANLL) leukemia. Expression of three components of telomerase as well as telomerase activity was found in all leukemic cells. Chemiluminescent detection of terminal restriction fragments (TRF) from DNA isolated from ALL cells showed variable patterns expressing considerable heterogeneity of telomere length. The ALL cells appeared to have both long and short telomere lengths, in contrast to normal peripheral lymphocytes, which produced limited pattern of TRF. The ANLL cells produced predominantly short telomere pattern despite high telomerase activity and expression. It can be concluded that high telomerase activity and expression in leukemic cells is not always correlated with long telomeres (TRF pattern).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia
/
Telômero
/
Telomerase
Limite:
Humans
Idioma:
En
Revista:
Int J Mol Med
Ano de publicação:
2003
Tipo de documento:
Article