Genotyping of clinical Rhodotorula mucilaginosa isolates by pulsed field gel electrophoresis.
Mycoses
; 46(11-12): 487-91, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14641622
ABSTRACT
Identification and typing of fungal isolates is a prerequisite for control and prevention of nosocomial infections. As the discriminatory power of phenotypic methods is not sufficient for epidemiological purposes, genotyping methods such as DNA fingerprinting, random amplification of polymorphic DNA (RAPD) analysis, or pulsed field gel electrophoresis (PFGE) are preferred. To our knowledge, this study is the first application of PFGE for typing Rhodotorula mucilaginosa strains. The PFGE patterns of six clinical isolates produced two different karyotypes, which were confirmed by RAPD analysis. Five strains isolated from bloodstream infections from three different institutions showed the same karyotype and RAPD patterns, while the urological specimen differed slightly.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rhodotorula
/
Técnicas de Tipagem Micológica
/
Eletroforese em Gel de Campo Pulsado
/
Micoses
Limite:
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Mycoses
Ano de publicação:
2003
Tipo de documento:
Article