Induced ovulation and egg deposition in the direct developing anuran Eleutherodactylus coqui.
Reprod Biol Endocrinol
; 2: 6, 2004 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-14748925
ABSTRACT
This study investigates ovulation and egg deposition behaviors in the anuran Eleutherodactylus coqui from Puerto Rico in response to stimulation with gonadotropin and gonadotropin releasing hormones. Five hormones were tested by injection over a range of doses, including mammalian LHRH, avian LHRH, fish LHRH, D-Ala6, des-Gly10 ethylamide LHRH and hCG. We report a low level of ovulation and egg deposition in response to all hormones, with the most complete and consistent results from the non-natural D-Ala6, des-Gly10 ethylamide LHRH derivative. To confirm the viability of eggs produced in this manner we performed in vitro fertilization experiments that resulted in the development of normal frogs. Reproductive behaviors in E. coqui are apparently not controlled by a mammalian form of LHRH as reported in other common laboratory anuran species. D-Ala6, des-Gly10 ethylamide LHRH induces ovulation and deposition of mature and fertilizable eggs in E. coqui.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Anuros
/
Oviposição
/
Ovulação
Limite:
Animals
País/Região como assunto:
Caribe
/
Puerto rico
Idioma:
En
Revista:
Reprod Biol Endocrinol
Ano de publicação:
2004
Tipo de documento:
Article