[Comparative proteomics research of apoptosis initiation induced by homoharringtonine in HL-60 cells].
Zhonghua Xue Ye Xue Za Zhi
; 24(12): 624-8, 2003 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-14761608
ABSTRACT
OBJECTIVE:
To study the related proteins of apoptosis initiation induced by homoharringtonine (HHT) in HL-60 cells.METHODS:
After establishment of an apoptosis initiation model induced by HHT in HL-60 cells, proteins of untreated and HHT treated HL-60 cells were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) two-dimensional electrophoresis respectively. The alteration protein spots were identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.RESULTS:
Proteomics analysis showed that proteins including MHC class I antigen, calbindin D-28K, chloride channel protein 6, oncoprotein 18, zinc finger protein Helios and apoptosis inhibitor like protein 2 were involved in apoptosis initiation induced by HHT.CONCLUSION:
The present study might conduce to the researches of HL-60 cells carcinogenesis and pave the way to exploit drug precursor related to HHT and initiation of apoptosis in HL-60 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Proteoma
/
Harringtoninas
/
Proteínas dos Microtúbulos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Xue Ye Xue Za Zhi
Ano de publicação:
2003
Tipo de documento:
Article