Glucocorticoid-inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells.
Insect Mol Biol
; 13(2): 205-11, 2004 Apr.
Article
em En
| MEDLINE
| ID: mdl-15056368
Inducible, vector-based, expression systems that allow fine control of transgene expression are gaining more and more use in fundamental research as well as in therapeutic applications. In an effort to develop a tightly regulated heterologous expression system for Drosophila Schneider 2 cells, three different inducible reporter constructs were compared. These comprised six copies of the glucocorticoid response element fused to one of three distinct types of Drosophila gene promoters: (1) a TATA-box containing, (2) a TATA-less and (3) a bidirectional core sequence. These were fused to a luciferase reporter gene. The promoter constructs displayed different basal as well as agonist-induced activities. The implications of the observations made are discussed in the context of promoter properties and of induction of genes that may be studied in Drosophila.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Genes Reporter
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Drosophila melanogaster
/
Vetores Genéticos
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Glucocorticoides
Limite:
Animals
Idioma:
En
Revista:
Insect Mol Biol
Ano de publicação:
2004
Tipo de documento:
Article