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Determination of pentoxifylline and its metabolites in human plasma by high-performance liquid chromatography with solid-phase extraction.
Mancinelli, A; Pace, S; Marzo, A; Martelli, E A; Passetti, G.
Afiliação
  • Mancinelli A; Drug Metabolism and Pharmacokinetic Department, Pomezia, Rome, Italy.
J Chromatogr ; 575(1): 101-7, 1992 Mar 13.
Article em En | MEDLINE | ID: mdl-1517286
ABSTRACT
A simple and reliable method for the determination of pentoxifylline and its main metabolites in human plasma has been developed using high-performance liquid chromatography. After selective solid-phase extraction, pentoxifylline, its metabolites and an internal standard, 7-(2'-chloroethyl)theophylline, were separated on a 5-micron LiChrospher 100 RP-18 column using water-dioxan-acetonitrile (876.56.5, v/v/v) acidified with acetic acid (0.5%, v/v) as the mobile phase. The analytes were detected at 275 nm. The lowest detectable concentration for all analytes was 25 ng/ml; the recovery was 85%. The assay has been successfully applied to analysis of these compounds in human plasma after administration of an oral dose of 400 mg of pentoxifylline to healthy volunteers.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pentoxifilina / Cromatografia Líquida de Alta Pressão Limite: Humans Idioma: En Revista: J Chromatogr Ano de publicação: 1992 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pentoxifilina / Cromatografia Líquida de Alta Pressão Limite: Humans Idioma: En Revista: J Chromatogr Ano de publicação: 1992 Tipo de documento: Article