Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes.

van Keulen, Geertje; Siebring, Jeroen; Rembacz, Krzysztof P; Hoogeveen, Martin; Tomczynska, Magdalena; Dijkhuizen, Lubbert

van Keulen, Geertje; Siebring, Jeroen; Rembacz, Krzysztof P; Hoogeveen, Martin; Tomczynska, Magdalena; Dijkhuizen, Lubbert.

Afiliação

van Keulen G; Department of Microbiology, Groningen Biotechnology and Biomolecular Science Institute (GBB), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands. g.van.Keulen@biol.rug.nl

J Microbiol Methods ; 58(1): 139-42, 2004 Jul.

Article em En | MEDLINE | ID: mdl-15177913

ABSTRACT

ABSTRACT

Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis.

Assuntos

RNA Bacteriano/isolamento & purificação; Streptomyces/genética; Northern Blotting; Desoxirribonuclease I/química; Guanidinas/química; Isotiocianatos/química; Fenol/química; Esporos Bacterianos; Streptomyces/química

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptomyces / RNA Bacteriano Idioma: En Revista: J Microbiol Methods Ano de publicação: 2004 Tipo de documento: Article

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