The Bacillus secretion stress response is an indicator for alpha-amylase production levels.
Lett Appl Microbiol
; 39(1): 65-73, 2004.
Article
em En
| MEDLINE
| ID: mdl-15189290
ABSTRACT
AIMS:
Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to alpha-amylase production. METHODS ANDRESULTS:
The expression of a transcriptional htrB-lacZ gene fusion, and the levels of alpha-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of alpha-amylase production.CONCLUSION:
Our observations show that the secretion stress response can serve as an indicator for alpha-amylase production levels. SIGNIFICANCE AND IMPACT OF STUDY Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Resposta ao Choque Térmico
/
Alfa-Amilases
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Lett Appl Microbiol
Ano de publicação:
2004
Tipo de documento:
Article