Molecular cloning and characterization of cDNA encoding fibrinolytic enzyme-3 from earthworm Eisenia foetida.
Acta Biochim Biophys Sin (Shanghai)
; 36(4): 303-8, 2004 Apr.
Article
em En
| MEDLINE
| ID: mdl-15253157
ABSTRACT
The earthworm fibrinolytic enzyme-3 (EFE-3, GenBank accession No AY438622), from the earthworm Eisenia foetida, is a component of earthworm fibrinolytic enzymes. In this study, cDNA encoding the EFE-3 was cloned by RT-PCR. The cDNA contained an open reading frame of 741 nucleotides, which encoded a deduced protein of 247 amino acid residues, including signal sequences. EFE-3 showed a high degree of homology to earthworm (Lumbricus rebullus) proteases F-III-1, F-III-2, and bovine trypsin. The recombinant EFE-3 was expressed in E. coli as inclusion bodies, and the gene encoding the native form of EFE-3 was expressed in COS-7 cells in the medium. Both the refolding product of inclusion bodies and the secreted protease could dissolve the artificial fibrin plate.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Oligoquetos
/
Endopeptidases
/
Clonagem Molecular
/
DNA Complementar
Limite:
Animals
Idioma:
En
Revista:
Acta Biochim Biophys Sin (Shanghai)
Ano de publicação:
2004
Tipo de documento:
Article