Antibodies and complement enhance binding and uptake of HIV-1 by human monocytes.
AIDS
; 6(1): 35-41, 1992 Jan.
Article
em En
| MEDLINE
| ID: mdl-1543564
ABSTRACT
OBJECTIVE:
To characterize antibody- and complement-mediated binding and uptake of HIV-1 by human monocytes.DESIGN:
The first step in the infection of the monocyte by HIV-1 is binding of the virus to the susceptible cell. Procedures were designed to assess the influence of anti-HIV-1 antibodies and complement on this binding, and to study the process of internalization following binding.METHODS:
Human monocytes were incubated with fluorescein-labelled purified HTLV-IIIB virions and human sera with high-titre anti-HIV-1 antibodies and/or complement. Binding and uptake of virus by the monocytes was measured as fluorescence per cell by flow cytometry.RESULTS:
Binding of purified HIV-1 to monocytes was increased by complement and, to a lesser extent, by anti-HIV-1 antibodies. Uptake of HIV-1 bound to the monocyte appeared to be mediated by antibodies and was increased further by the presence of complement. Complement alone, however, resulted in the uptake of only a small part of the bound virus.CONCLUSIONS:
Complement significantly increases the binding of HIV-1 to human monocytes, and a combination of antibodies and complement efficiently mediates uptake of HIV-1 by monocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas do Sistema Complemento
/
Monócitos
/
HIV-1
Limite:
Humans
Idioma:
En
Revista:
AIDS
Ano de publicação:
1992
Tipo de documento:
Article