Inhibition of 5-lipoxygenase induces cell death in anti-inflammatory fatty acid-treated HL-60 cells.
JPEN J Parenter Enteral Nutr
; 28(5): 308-14, 2004.
Article
em En
| MEDLINE
| ID: mdl-15449569
ABSTRACT
BACKGROUND:
Enteral nutrition containing eicosapentaenoic (205 omega-3) and gamma-linolenic acid (183 omega-6) decreases leukotriene B4 levels and neutrophils in bronchoalveolar lavage fluid of patients and animals with acute respiratory distress syndrome. Reduction in pulmonary inflammation may be caused by decreased neutrophil migration or survival. We showed that apoptosis increases in eicosapentaenoic/gamma-linolenic acid-treated HL-60 cells. We hypothesize that eicosapentaenoic/gamma-linolenic acid-induced apoptosis involves downstream metabolic products of lipoxygenase and cyclooxygenase enzymes. This study determined the effects of inhibitors of lipoxygenase and cyclooxygenase enzymes on eicosapentaenoic/gamma-linolenic acid-treated HL-60 cells.METHODS:
Cells were incubated with 50 microM eicosapentaenoic/20 microM gamma-linolenic acid in the presence of an enzyme inhibitor (1-10 microM) for 12 hours. Compounds were used to inhibit cyclooxygenase (ibuprofen), 12-lipoxygenase (baicalein), or 5-lipoxygenase (AA-861). Flow cytometry assessed viability, apoptosis, and necrosis.RESULTS:
5-Lipoxygenase inhibition decreased cell viability and increased cell death (apoptosis + necrosis) in eicosapentaenoic/gamma-linolenic acid-treated HL-60 cells. Inhibition of cyclooxygenase 1 and 2 and 12-lipoxygenase had no significant effect on cellular viability and death in eicosapentaenoic/gamma-linolenic acid-treated HL-60 cells. Adding leukotriene B4 counteracted the effect of 5-lipoxygenase inhibition on apoptosis in eicosapentaenoic/gamma-linolenic acid-treated HL-60 cells.CONCLUSIONS:
These data suggest that the processing of eicosapentaenoic and gamma-linolenic acid by 5-lipoxygenase is critical to HL-60 cell survival.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Eicosapentaenoico
/
Inibidores de Lipoxigenase
/
Nutrição Enteral
/
Apoptose
/
Ácido gama-Linolênico
Limite:
Humans
Idioma:
En
Revista:
JPEN J Parenter Enteral Nutr
Ano de publicação:
2004
Tipo de documento:
Article