Separation, pre-concentration, and HPLC analysis of methylxanthines in urine samples.
J Sep Sci
; 27(14): 1166-72, 2004 Oct.
Article
em En
| MEDLINE
| ID: mdl-15537072
ABSTRACT
An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1,7-dimethyluric acid, and 1,3,7-trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP-18 (7 microm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Urina
/
Xantinas
/
Cromatografia Líquida de Alta Pressão
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2004
Tipo de documento:
Article