[Research on the growth inhibition of T47D cell and reversion of p16 hypermethylation by CDP].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 35(6): 792-6, 2004 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15573756
ABSTRACT
OBJECTIVE:
To inquire into the mechanism of CDP (a purified component of Chinese crude drug) in inhibiting the breast cancer T47D cell growth.METHODS:
T47D cells were treated with different concentration of 5-azacytidine (5-aza-CR) and CDP for several days. The growth rate was assessed by cell proliferation experiment (MTT colorimetric assay). The changes in apoptic peak and cell cycle distribution were detected by flow cytometry (FCM). The levels of methylation and unmethylation status of p16 were detected by methylation specific PCR (MSP).RESULTS:
After treatment with the two drugs, the cell growth rate decreased in a dose and time dependent manner (P<0.05). The cell cycle was influenced by the well-chosen concentration of 5-aza-CR (2 micromol/L) and CDP (50 micromol/L) the cell number increased from 65.1% to 71.3%, 84.3% in G0/G1 phase and decreased from 19.4% to 14.3%, 7.2% in S phase. Demethylation on p16 gene occurred after treatment with any of the two drugs for 6 days.CONCLUSION:
CDP can reverse p16 hypermethylation and may hence inhibit the proliferation of tumor cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Medicamentos de Ervas Chinesas
/
Metilação de DNA
/
Genes p16
/
Antineoplásicos Fitogênicos
Limite:
Female
/
Humans
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2004
Tipo de documento:
Article