Molecular cloning of orangutan and gibbon MHC class I cDNA. The HLA-A and -B loci diverged over 30 million years ago.
J Immunol
; 148(8): 2547-54, 1992 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-1560209
ABSTRACT
To investigate whether the classical HLA MHC class I loci have been preserved during evolution of the primates, we have cloned, sequenced, and expressed eight MHC class I cDNA from orangutan and gibbon lymphocytes. Both the HLA-A and -B loci are present in both of these species. In fact, lymphocytes from the orangutan expressed three HLA-B-related gene products, suggesting that the ancestral homologue of the HLA-B locus had undergone a duplication in this species. Interestingly, several amino acid motifs thought to be important in the Ag-presenting function of MHC class I molecules were preserved in the Ag-recognition sites of the orangutan and gibbon MHC class I molecules. Finally, these findings suggest that the recombination event between the HLA-A and -E loci occurred over 38 million years ago. These data indicate that the HLA-A and -B loci are extremely stable and that recombination between them is rare. Furthermore, the data presented here argue against the role of concerted evolution in the evolution of primate MHC class I molecules.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pongo pygmaeus
/
DNA
/
Genes MHC Classe I
/
Antígenos HLA-A
/
Antígenos HLA-B
/
Mapeamento Cromossômico
/
Clonagem Molecular
/
Hylobates
Limite:
Animals
Idioma:
En
Revista:
J Immunol
Ano de publicação:
1992
Tipo de documento:
Article