Apoptotic death of infiltrating cells in human cardiac allografts is regulated by IL-2, FASL, and FLIP.
Transplant Proc
; 36(10): 3143-8, 2004 Dec.
Article
em En
| MEDLINE
| ID: mdl-15686714
ABSTRACT
INTRODUCTION:
In vitro studies have shown that apoptotic cell death is triggered by a IL-2-dependent activation of the Fas-FasL pathway and that this pathway can be inhibited by FLIP.METHODS:
To define whether FLIP regulates apoptotic death of graft infiltrating T-cells during IL-2-mediated rejection, we analyzed endomyocardial biopsies (EMB) from cardiac allograft recipients for CD3, DNA strand breaks (TUNEL assay), FLIP (mRNA and protein), and FasL mRNA expression.RESULTS:
Apoptosis was present in CD3+ T-cell infiltrates. The number of TUNEL-stained mononuclear cells was inversely correlated with FLIP mRNA expression levels (P=.09). FLIP protein was present in 5% to 10% of the infiltrating cells and was constitutively produced by cardiomyocytes irrespective of the rejection grade. Rejection biopsies had elevated IL-2 and FasL mRNA expression levels compared to the expression levels before and after acute rejection (P=.03 and P=.11), while FLIP mRNA expression levels were significantly decreased during rejection (P=.05).CONCLUSION:
Our results indicate that during the IL-2-induced rejection process, infiltrated T cells become more sensitive to apoptosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Proteínas
/
Transplante de Coração
/
Interleucina-2
/
Apoptose
Limite:
Humans
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2004
Tipo de documento:
Article