Truncated midkine induces transformation of cultured cells and short latency of tumorigenesis in nude mice.
Cancer Lett
; 219(1): 83-9, 2005 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-15694667
ABSTRACT
To test whether truncated midkine (tMK), an alternative spliced form of exon 3, induces the transformation of mammalian cells, tMK cDNA was transfected into SW-13 cells. Although, the growth of MK transfectant (SW-13/MK) cells was close to those of the parent SW-13 and vector transfectant (SW-13/empty) cells, the growth of tMK transfectant (SW-13/tMK) cells was significantly promoted compared with that of the above three cells. Both SW-13/tMK and SW-13/MK formed colonies in 0.35% soft agar, indicating that tMK and MK induce mammalian cell transformation. SW-13/tMK frequently formed solid tumor after its subcutaneous injection into nude mice. Additionally, in SW-13/tMK and SW-13/MK-injected mice, advanced visible tumors were detected compared with that in the case of SW-13/empty-injected mice as control. These findings indicate that tMK induces mammalian cell transformation and promotes tumor establishment in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transformação Celular Neoplásica
/
Citocinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cancer Lett
Ano de publicação:
2005
Tipo de documento:
Article