Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR.
Appl Environ Microbiol
; 71(4): 2190-4, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15812058
ABSTRACT
A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
Biofilmes
/
Listeria monocytogenes
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2005
Tipo de documento:
Article