Spectrophotometric determination of DNA using a near infrared probe 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine.
Spectrochim Acta A Mol Biomol Spectrosc
; 61(9): 2103-7, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-15911398
ABSTRACT
A direct spectrophotometric determination of DNA in solution has been developed using a near infrared probe, 1,1'-disulfobutyl-3,3,3',3'-tetramethylindotricarbocyanine (DSTCY). In pH = 7.5 Tris-HCl buffer, the reaction of DSTCY with DNA was complete within 10 min at room temperature in the presence of cetyltrimethylammonium bromide (CTAB), which leaded to a sharp increase of the absorbance at 474 nm. The concentration of DNA can be determined in such wavelength with the linear range of 0.5-8.0 microg ml(-1) and the detection limit of 45 ng ml(-1).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Carbocianinas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Ano de publicação:
2005
Tipo de documento:
Article