Grape-seed derived procyanidins interfere with adipogenesis of 3T3-L1 cells at the onset of differentiation.
Int J Obes (Lond)
; 29(8): 934-41, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-15917849
ABSTRACT
OBJECTIVE:
Our group's previous results on the effects of a grape seed procyanidin extract (GSPE) on adipose metabolism showed that peroxisome proliferator-activated receptor-gamma (PPARgamma) plays a central role in the lipolytic effects of GSPE on adipocytes. Since PPARgamma2 is a main regulator of the differentiation process of adipocytes, we investigated whether GSPE affects the adipogenesis of 3T3-L1 cells.DESIGN:
We performed a time point screening by treating 3T3-L1 cells with GSPE during the differentiation process for 24 h. MEASUREMENTS Differentiation markers and differential gene expression due to GSPE treatment (using the microarray technique).RESULTS:
Twenty four hour-GSPE treatment at the onset of differentiation reduces adipose-specific markers and maintains the expression of preadipocyte marker preadipocyte factor-1 (Pref-1) significantly elevated. These effects were not found in other time points. Microarray analysis of gene expression after GSPE treatment at the early stage of differentiation showed a modified gene expression profile in which cell cycle and growth-related genes were downregulated by GSPE.CONCLUSION:
These results suggest that GSPE affects adipogenesis, mainly at the induction of differentiation, and that procyanidins may have a new role in which they impede the formation of adipose cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Catequina
/
Adipócitos
/
Vitis
/
Biflavonoides
/
Proantocianidinas
/
Antioxidantes
Limite:
Animals
Idioma:
En
Revista:
Int J Obes (Lond)
Ano de publicação:
2005
Tipo de documento:
Article