Epitope analysis of insulin autoantibodies using recombinant Fab.
Clin Exp Immunol
; 140(3): 564-71, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15932520
ABSTRACT
Autoantibodies to insulin are often the first autoantibodies detected in young children with type 1 diabetes and can be present before the onset of clinical diabetes. These autoantibodies and their epitopes are, however, not well characterized. We explored the use of monoclonal antibodies and their recombinant Fab as reagents for epitope analysis. In this study we cloned and characterized the recombinant Fab of the insulin-specific monoclonal antibody CG7C7. We found the epitope of this antibody to be located predominantly at the A-chain loop of the insulin molecule. The recombinant Fab was then used to compete for insulin binding against insulin autoantibodies present in sera from patients with type 1 or type 1.5 diabetes. In competition experiments with sera positive for autoantibodies to insulin the recombinant Fab significantly reduced the binding to [125I]-insulin by sera of type 1 (n = 35) and type 1.5 diabetes [latent autoimmune diabetes in adults (LADA)] (n = 14) patients (P < 0.0001). We conclude that competition between insulin-specific monoclonal antibodies or their recombinant Fab and insulin autoantibodies should prove useful in the epitope analysis of autoantibodies to insulin.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estado Pré-Diabético
/
Autoanticorpos
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Fragmentos Fab das Imunoglobulinas
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Diabetes Mellitus Tipo 1
/
Anticorpos Anti-Insulina
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Anticorpos Monoclonais
/
Epitopos
Limite:
Adolescent
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Adult
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Child
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Child, preschool
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Female
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Humans
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Infant
/
Male
Idioma:
En
Revista:
Clin Exp Immunol
Ano de publicação:
2005
Tipo de documento:
Article