RAIDD is required for apoptosis of PC12 cells and sympathetic neurons induced by trophic factor withdrawal.
Cell Death Differ
; 13(1): 75-83, 2006 Jan.
Article
em En
| MEDLINE
| ID: mdl-15947787
ABSTRACT
Caspase 2 has been implicated in trophic deprivation-induced neuronal death. We have shown that overexpression of the caspase 2-binding protein RAIDD induces neuronal apoptosis, acting synergistically with trophic deprivation. Currently, we examine the role of endogenous RAIDD in apoptosis of PC12 cells and sympathetic neurons. Expression of a truncated caspase recruitment domain-only form of caspase 2, which presumably disrupts the RAIDD interaction with endogenous caspase 2, attenuated trophic deprivation-induced apoptosis. Furthermore, downregulation of RAIDD by small interfering RNA led to inhibition of trophic deprivation-induced death, whereas death induced by DNA damage, which is not caspase 2-mediated, was not inhibited. Therefore, RAIDD, likely through interaction with caspase 2, is involved in trophic deprivation-induced neuronal apoptosis. This is the first demonstration of the involvement of RAIDD in apoptosis, and provides further support for the idea that apoptotic pathways in the same system may differ depending on the initiating stimulus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Proteínas Adaptadoras de Transdução de Sinal
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
Cell Death Differ
Ano de publicação:
2006
Tipo de documento:
Article