Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin.
FEBS Lett
; 579(18): 3885-90, 2005 Jul 18.
Article
em En
| MEDLINE
| ID: mdl-15987637
ABSTRACT
Calmodulin (CaM), a key Ca(2+) sensor in eukaryotes, regulates diverse cellular processes by interacting with many proteins. To identify Ca(2+)/CaM-mediated signaling components, we screened an Arabidopsis expression library with horseradish peroxidase-conjugated Arabidopsis calmodulin2 (AtCaM2) and isolated a homolog of the UBP6 deubiquitinating enzyme family (AtUBP6) containing a Ca(2+)-dependent CaM-binding domain (CaMBD). The CaM-binding activity of the AtUBP6 CaMBD was confirmed by CaM mobility shift assay, phosphodiesterase competition assay and site-directed mutagenesis. Furthermore, expression of AtUBP6 restored canavanine resistance to the Deltaubp6 yeast mutant. This is the first demonstration that Ca(2+) signaling via CaM is involved in ubiquitin-mediated protein degradation and/or stabilization in plants.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Calmodulina
/
Arabidopsis
/
Proteínas de Arabidopsis
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2005
Tipo de documento:
Article