Transcriptional profiles and structural models of the Synechocystis sp. PCC 6803 Deg proteases.
Photosynth Res
; 84(1-3): 57-63, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-16049755
ABSTRACT
The Synechocystis sp. PCC 6803 genome harbours a deg gene family consisting of three members, degP (htrA, slr1204), degQ (hhoA, sll1679) and degS (hhoB, sll1427). We studied the environmental regulation of the Synechocystis sp. PCC 6803 deg genes at the level of transcription and protein structures of the gene products to evaluate their hypothetical role in D1 protein turnover. Northern blotting showed that transcription of the deg genes is differentially regulated, supporting a view of distinct roles of Degs in cellular processes. The oligomerization state as well as the three dimensional structures of the Synechocystis sp. PCC 6803 Deg proteases were predicted based on an amino acid sequence alignment and comparison of the Deg crystal structures from human, Escherichia coli and Thermotoga maritima. The structures of the Synechocystis sp. PCC 6803 Degs resemble more the Thermotoga maritima Deg enzyme structure than the Escherichia coli one. Moreover, the structures of the LA-loops hint towards a homotrimeric form of the Synechocystis sp. PCC 6803 Deg proteases.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
/
Transcrição Gênica
/
Regulação Bacteriana da Expressão Gênica
/
Synechocystis
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Photosynth Res
Ano de publicação:
2005
Tipo de documento:
Article