Comparison of zirconia- and silica-based reversed stationary phases for separation of enkephalins.
J Chromatogr A
; 1087(1-2): 104-11, 2005 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-16130703
ABSTRACT
In this study, the separation of biologically active peptides on two zirconia-based phases, polybutadiene (PBD)-ZrO2 and polystyrene (PS)-ZrO2, and a silica-based phase C18 was compared. Basic differences in interactions on both types of phases led to quite different selectivity. The retention characteristics were investigated in detail using a variety of organic modifiers, buffers, and temperatures. These parameters affected retention, separation efficiency, resolution and symmetry of peaks. Separation systems consisting of Discovery PBD-Zr column and mobile phase composed of a mixture of acetonitrile and phosphate buffer, pH 2.0 (4555, v/v) at 70 degrees C and Discovery PS-Zr with acetonitrile and phosphate buffer, pH 3.5 in the same (v/v) ratio at 40 degrees C were suitable for a good resolution of enkephalin related peptides. Mobile phase composed of acetonitrile and phosphate buffer, pH 5.0 (2278, v/v) was appropriate for separation of enkephalins on Supelcosil C18 stationary phase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Zircônio
/
Encefalinas
/
Dióxido de Silício
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2005
Tipo de documento:
Article