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A DNA polymerase from the archaeon Sulfolobus solfataricus shows sequence similarity to family B DNA polymerases.
Pisani, F M; De Martino, C; Rossi, M.
Afiliação
  • Pisani FM; Istituto di Biochimica delle Proteine ed Enzimologia, Consiglio Nazionale Ricerche, Naples, Italy.
Nucleic Acids Res ; 20(11): 2711-6, 1992 Jun 11.
Article em En | MEDLINE | ID: mdl-1614858
The gene encoding the thermostable DNA polymerase from the archaeon Sulfolobus solfataricus (strain MT 4) was isolated by means of two degenerate oligonucleotide probes. They were designed on the basis of partial enzyme amino acid sequences. The gene was found to encode a 882 residues polypeptide chain with a deduced molecular mass of about 100 kDa. By comparison with other archaeal genes, putative regulatory sites were identified in the gene-flanking regions. By computer-assisted homology search, several sequence similarities among S. solfataricus and family B DNA polymerases were found. In addition, conserved sequence motifs, implicated in the 3'-5' exonuclease activity of E. coli DNA polymerase I and shared by various family A and B DNA polymerases, were also identified. This result suggests that the proofreading domains of all these enzymes are evolutionarily related.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfolobus / DNA Polimerase Dirigida por DNA / Genes Bacterianos Idioma: En Revista: Nucleic Acids Res Ano de publicação: 1992 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfolobus / DNA Polimerase Dirigida por DNA / Genes Bacterianos Idioma: En Revista: Nucleic Acids Res Ano de publicação: 1992 Tipo de documento: Article