Direct activation of gelatinase B (MMP-9) by hay dust suspension and different components of organic dust.
Vet Immunol Immunopathol
; 109(3-4): 289-95, 2006 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-16181685
ABSTRACT
Matrix metalloproteinases (MMPs) are involved in tissue destruction in allergic airway diseases. We studied the ability of various allergenic substances to directly activate recombinant 92kDa proMMP-9. The substances included hay dust suspension (HDS) and its components (supernatant, particulate matter and wash fluid of particulate matter), storage mite extract and two Aspergillus fumigatus extracts. The allergen suspensions were incubated in vitro with proMMP-9. After incubation the conversion of proMMP-9 to 10kDa lower active forms were studied using gelatin zymography and Western immunoblot quantified by computerized densitometry. All studied allergens except HDS significantly and efficiently activated proMMP-9 as compared to a negative control. At the concentrations employed, the most potent activators were A. fumigatus extracts and mite suspension. The greater potency of mite and fungi as proMMP-9 activators suggests that these allergens may be more damaging to airways even at low concentrations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colagenases
/
Precursores Enzimáticos
/
Doenças dos Cavalos
/
Hipersensibilidade
Limite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Ano de publicação:
2006
Tipo de documento:
Article