Heat-labile enterotoxin crystal forms with variable A/B5 orientation. Analysis of conformational flexibility.
FEBS Lett
; 305(2): 81-5, 1992 Jun 29.
Article
em En
| MEDLINE
| ID: mdl-1618345
ABSTRACT
A new native crystal form of heat-labile enterotoxin (LT) has two AB5 complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with other crystal forms of LT shows that there is considerable conformational freedom for orientating the A subunit with respect to the B pentamer. The rotations of A in different crystal forms do not follow one specific axis, but most of them share a hinge point, close to the main interaction area between A and B5. Analysis of the two high-resolution structures available shows that these rotations cause very little change in the actual interactions between A and B5.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Proteínas de Escherichia coli
/
Enterotoxinas
/
Escherichia coli
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1992
Tipo de documento:
Article