S-carboxymethylcysteine normalises airway responsiveness in sensitised and challenged mice.
Eur Respir J
; 26(4): 577-85, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16204586
ABSTRACT
S-carboxymethylcysteine (S-CMC) has been used as a mucoregulator in respiratory diseases. However, the mechanism of action of S-CMC on allergic airway inflammation has not yet been defined. In the present study, BALB/c mice were initially sensitised and challenged to ovalbumin (OVA) and, weeks later, re-challenged with OVA (secondary challenge). S-CMC (5-100 mg.kg-1) was administered from 2 days before the secondary challenge through to the day of assay. Mice developed airway hyperresponsiveness (AHR) 6 h after the secondary challenge and increased numbers of neutrophils were present in the bronchoalveolar lavage (BAL) fluid. At 72 h after secondary challenge, mice again developed AHR, but the BAL fluid contained large numbers of eosinophils. S-CMC treatment was found to reduce AHR and neutrophilia at 6 h, as well as eosinophilia and AHR at 72 h. These effects appeared to be dose dependent. Goblet cell hyperplasia, observed at 72 h, was reduced by S-CMC. In BAL fluid, increased levels of interferon-gamma, interleukin (IL)-12 and IL-10 and decreased levels of IL-5 and IL-13 were detected. In conclusion, the data indicate that S-carboxymethylcysteine is effective in reducing airway hyperresponsiveness and airway inflammation at two distinct phases of the response to the secondary allergen challenge in sensitised mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbocisteína
/
Hiper-Reatividade Brônquica
/
Anti-Inflamatórios
Limite:
Animals
Idioma:
En
Revista:
Eur Respir J
Ano de publicação:
2005
Tipo de documento:
Article