Different signaling pathways inhibit DNA methylation activity and up-regulate IFN-gamma in human lymphocytes.
J Leukoc Biol
; 78(6): 1339-46, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16204617
ABSTRACT
DNA methylation is recognized increasingly for its prominent role in controlling diverse immune processes. In this study, we show that in Jurkat T cells and fresh peripheral lymphocytes, short-time incubation with protein kinase C activators or phosphatase inhibitors down-regulate DNA methylation activity in a dose-dependent manner. This inhibition correlates with the induction of the interferon-gamma (IFN-gamma) gene, which contains several CG sequences in its promoter. The expression of mRNA and protein of the different DNA methyltransferases did not decrease after the treatment. In addition, sulfydryl reagents have a strong inhibitory effect on DNA methylation activity and also induce IFN-gamma gene expression, thus suggesting a link between both effects.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Linfócitos
/
Transdução de Sinais
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Interferon gama
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Metilação de DNA
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Imunidade Celular
Idioma:
En
Revista:
J Leukoc Biol
Ano de publicação:
2005
Tipo de documento:
Article