[Effects of selenium and B-27 supplements on viability and differentiation of neural stem cell in newborn rat].
Zhonghua Yu Fang Yi Xue Za Zhi
; 39(5): 324-7, 2005 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-16266543
ABSTRACT
OBJECTIVE:
To assess how trace element selenium and B27 supplements affect the neural stem cell (NSc) differentiation in vitro.METHODS:
The development and differentiation of NSc from the newborn rat were observed with primary culture and subculture during treating by sodium-selenite, and selenium-methyl-cysteine (SMC). The immunocytochemistry techniques were used to identify the NSc and mature protein expression with neuron marker beta-tubulin, astrocyte marker GFAP, and oligodendrocyte marker CNPase. The neurosphere morphology and neurite outgrowth were observed.RESULTS:
Adding the complete B-27 serum-free supplement, Selenium could promote the neurosphere viability, development and differentiation. Without selenium and B-27, neurosphere could not survive and differentiate. Without B-27 in the medium but there containing selenium, the neurosphere could promote the viability and development into neuron, astrocyte and oligodendrocyte, as compared with the no-containing B-27 and selenium groups, these differentiated cells might have more quantity, more branches and better morphological nerve net. The count of the neuron, astrocyte and oligodendrocyte was 11.2/Hp, 16.1/Hp and 9.3/Hp.CONCLUSIONS:
The selenium should be very important for neural stem cells' survival. Selenium could promote the neurosphere cells differentiation and development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Selênio
/
Células-Tronco
/
Diferenciação Celular
/
Neurônios
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Ano de publicação:
2005
Tipo de documento:
Article