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Preparation and development of equine hyperimmune globulin F(ab')2 against severe acute respiratory syndrome coronavirus.
Lu, Jia-Hai; Guo, Zhong-Min; Han, Wen-Yu; Wang, Guo-Ling; Zhang, Ding-Mei; Wang, Yi-Fei; Sun, Sheng-Yun; Yang, Qin-He; Zheng, Huan-Ying; Wong, Bing L; Zhong, Nan-Shan.
Afiliação
  • Lu JH; Sun Yat-sen University, Guangzhou 510080, China. jiahailu@yahoo.com.cn
Acta Pharmacol Sin ; 26(12): 1479-84, 2005 Dec.
Article em En | MEDLINE | ID: mdl-16297347
AIM: The resurgence of severe acute respiratory syndrome (SARS) is still a threat because the causative agent remaining in animal reservoirs is not fully understood, and sporadic cases continue to be reported. Developing high titers of anti-SARS hyperimmune globulin to provide an alternative pathway for emergent future prevention and treatment of SARS. METHODS: SARS coronavirus (CoV)F69 (AY313906) and Z2-Y3 (AY394989) were isolated and identified from 2 different Cantonese onset SARS patients. Immunogen was prepared from SARS-CoV F69 strain. Six health horses were immunized 4 times and serum was collected periodically to measure the profile of specific IgG and neutralizing antibodies using indirect enzyme-linked immunosorbent assay and a microneutralization test. Sera were collected in large amounts at the peak, where IgG was precipitated using ammonium sulphate and subsequently digested with pepsin. The product was then purified using anion-exchange chromatography to obtain F(ab')2 fragments. RESULTS: The specific IgG and neutralizing antibody titers peaked at approximately week 7 after the first immunization, with a maximum value of 1:14210. The sera collected at the peak were then purified. Fragment of approximately 15 g F(ab')2 was obtained from 1litre antiserum and the purity was above 90% with the titer of 1:5120, which could neutralize the other strain (SARS-CoV Z2-Y3) as well. CONCLUSION: This research provides a viable strategy for the prevention and treatment of SARS coronavirus infection with equine hyperimmune globulin, with the purpose of combating any resurgence of SARS.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Fragmentos Fab das Imunoglobulinas / Coronavírus Relacionado à Síndrome Respiratória Aguda Grave / Soros Imunes Limite: Animals / Humans Idioma: En Revista: Acta Pharmacol Sin Ano de publicação: 2005 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Fragmentos Fab das Imunoglobulinas / Coronavírus Relacionado à Síndrome Respiratória Aguda Grave / Soros Imunes Limite: Animals / Humans Idioma: En Revista: Acta Pharmacol Sin Ano de publicação: 2005 Tipo de documento: Article